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Dual action of epidermal growth factor: extracellular signal-stimulated nuclear--cytoplasmic export and coordinated translation of selected messenger RNA
- Source :
- The Journal of Cell Biology. Feb 8, 2010, Vol. 188 Issue 3, p325, 9 p.
- Publication Year :
- 2010
-
Abstract
- We report the first example of a coordinated dual action of epidermal growth factor (EGF) in stimulating the nuclear--cytoplasmic export and translation of a select messenger RNA (mRNA). The effect of EGF is mediated by the RNA-binding protein Grb7 (growth factor receptor-bound protein 7), which serves as an adaptor for a specific mRNA--protein export complex and a translational regulator. Using the [kappa]--opioid receptor (OR [KOR]) as a model, we demonstrate that EGF activates nuclear SHP-2 (Src homology region 2--containing tyrosine phosphatase), which dephosphorylates Grb7 in the nucleus. Hypophosphorylated Grb7 binds to the KOR mRNA and recruits the Hu antigen R--exportin-1 (CRM1) complex to form a nuclear--cytoplasmic export complex that exports KOR mRNA. EGF also activates focal adhesion kinase in the cytoplasm to rephosphorylate Grb7, releasing KOR mRNA for active translation. In summary, this study uncovers a coordinated, dual activity of EGF in facilitating nuclear export of a specific mRNA--protein complex as well as translational activation of the exported mRNA. doi/10.1083/jcb.200910083
Details
- Language :
- English
- ISSN :
- 00219525
- Volume :
- 188
- Issue :
- 3
- Database :
- Gale General OneFile
- Journal :
- The Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.224404991