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Zscan4 regulates telomere elongation and genomic stability in ES cells
- Source :
- Nature. April 8, 2010, Vol. 464 Issue 7290, p858, 8 p.
- Publication Year :
- 2010
-
Abstract
- Exceptional genomic stability is one of the hallmarks of mouse embryonic stem (ES) cells. However, the genes contributing to this stability remain obscure. We previously identified Zscan4 as a specific marker for two-cell embryo and ES cells. Here we show that Zscan4 is involved in telomere maintenance and long-term genomic stability in ES cells. Only 5% of ES cells express Zscan4 at a given time, but nearly all ES cells activate Zscan4 at least once during nine passages. The transient Zscan4-positive state is associated with rapid telomere extension by telomere recombination and upregulation of meiosis-specific homologous recombination genes, which encode proteins that are colocalized with ZSCAN4 on telomeres. Furthermore, Zscan4 knockdown shortens telomeres, increases karyotype abnormalities and spontaneous sister chromatid exchange, and slows down cell proliferation until reaching crisis by passage eight. Together, our data show a unique mode of genome maintenance in ES cells.<br />Mouse ES cells are derived from the inner cell mass (ICM) of blastocysts (1,2), and have similar gene expression patterns to ICM cells (3). The defining features of ES cells [...]
- Subjects :
- Genetic markers -- Identification and classification
Genomics -- Research
Embryonic development
Embryonic stem cells -- Genetic aspects -- Growth
Telomeres -- Properties
Genetic recombination -- Methods
Company growth
Environmental issues
Science and technology
Zoology and wildlife conservation
Subjects
Details
- Language :
- English
- ISSN :
- 00280836
- Volume :
- 464
- Issue :
- 7290
- Database :
- Gale General OneFile
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.223750914
- Full Text :
- https://doi.org/10.1038/nature08882