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GyrA interacts with MarR to reduce repression of the marRAB operon in Escherichia coli
- Source :
- Journal of Bacteriology. Feb, 2010, Vol. 192 Issue 3-4, p942, 7 p.
- Publication Year :
- 2010
-
Abstract
- Bacterial two-hybrid studies of randomly cloned Escherichia coli DNA identified a physical interaction between GyrA, subunit A of gyrase, and MarR, a repressor of the marRAB operon. GyrA-His immobilized on Ni-nitrilotriacetic acid (NiNTA) resin bound MarR, while MarR alone did not bind. GyrA interfered with MarR binding to marO, as detected by electrophoretic mobility assays. In a strain bearing the marRAB operon and a marO-lacZ reporter, overexpression of GyrA increased LacZ activity, indicating decreased repression of marO-lacZ by MarR. These results were confirmed by an increased survival of cells treated with quinolones and other antibiotics when GyrA was overexpressed. This work, like a previous study examining TktA (12), shows that unrelated proteins can regulate MarR activity. The findings reveal an unexpected regulatory function of GyrA in antibiotic resistance. doi: 10.1128/JB.01259-09
Details
- Language :
- English
- ISSN :
- 00219193
- Volume :
- 192
- Issue :
- 3-4
- Database :
- Gale General OneFile
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.220201502