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Mapping of the signal peptide-binding domain of Escherichia coli SecA using Forster resonance energy transfer

Authors :
Auclair, Sarah M.
Moses, Julia P.
Musial-Siwek, Monika
Kendall, Debra A.
Oliver, Donald B.
Mukerji, Ishita
Source :
Biochemistry. Feb 2, 2010, Vol. 49 Issue 4, 782-792
Publication Year :
2010

Abstract

Forster resonance energy transfer methodology was employed to map the location of the SecA signal peptide-binding domain using a collection of functional monocysteine SecA mutants and alkaline phosphatase signal peptides labeled with appropriate donor-acceptor fluorophores. The identified domain region by FRET was found to be located at a multidomain interface within the heart of SecA, surrounded by and potentially responsive to domains important for binding nucleotide, mature portions of the preprotein, and the SecYEG channel.

Details

Language :
English
ISSN :
00062960
Volume :
49
Issue :
4
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.219702229