Back to Search
Start Over
Impact of genome reduction on bacterial metabolism and its regulation
- Source :
- Science. Nov 27, 2009, Vol. 326 Issue 5957, p1263, 6 p.
- Publication Year :
- 2009
-
Abstract
- To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplosma pneumonioe. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and [sup.133]C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however. 10.1126/science.1177263
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 326
- Issue :
- 5957
- Database :
- Gale General OneFile
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.214526992
- Full Text :
- https://doi.org/10.1126/science.1177263