Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the cholorocatechol degradative genes from Pseudomonas sp. strain B13

A study was undertaken to determine the unknown characteristics of the transmissible chlorocatechol degradation (clc) gene from the Pseudomonas specie strain B13 utilizing pulse-field gel eletrophoresis, DNA-DNA hybridizations and cosmid mapping. The B13 strain was considered a pseudomonad using the 3-chlorobenzoate as the main source of carbon and energy. Results revealed that clc genes were discovered on a transmissible element and the physical appearance of this element was detected by its tandem amplification in the host strain, P. putida F1.