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Misincorporation of 2'-deoxyoxanosine 5'-triphosphate by DNA polymerases and its implication for mutagenesis

Authors :
Suzuki, Toshinori
Yoshida, Mitsuo
Yamada, Masaki
Ide, Hiroshi
Kobayashi, Mutsumi
Kanaori, Kenji
Tajima, Kunihiko
Makino, Keisuke
Source :
Biochemistry. August 18, 1998, Vol. 37 Issue 33, p11592, 7 p.
Publication Year :
1998

Abstract

A study was conducted to characterize the formation of 2-deoxyoxanosine 5'-triphosphate (dOTP) from 2'-deoxyguanosine 5'-triphosphate (dGTP) by HNO2 treatment. The study also analyzed the misincorporation of dOTP by DNA polymerases during the replication of M13mp18 and oligodeoxynucleotide templates. Results indicated that the nitrous acid treatment of dGTP in solution supported the synthesis of dOTP and 2'-deoxyxanthosine 5'-triphosphate.

Details

ISSN :
00062960
Volume :
37
Issue :
33
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.21125954