Photoinhibition of photosynthesis in vivo results in singlet oxygen production detection via nitroxide-induced fluorescence quenching in broad bean leaves

A study was conducted to examine the production of singlet oxygen in bean leaves supporting ambiguous electron paramagnetic resonance measurements. Singlet oxygen was determined in bean leaves which were saturated with DanePy by observing the fluorescence quenching of DanePy. Results indicated that lincomycin hindered photoinhibition and the synthesis of proteins. Findings also showed that less single oxygen was obtained after 50% of the inactive photosystem II centers were determined.