Characterization of PKA isoforms and kinase-dependent activation of chloride secretion in T84 cells

Research was conducted to determine which cAMP-dependent protein kinase (PKA) isoforms are present in the human colonic epithelial cell line T84, a well-characterized model cell line of transepithelial chloride secretion. Site-selective cAMP analogs were utilized to synergistically activate PKA I and PKA II in intact T84 cells aimed at elucidating the influence of each isozyme as a modulator of chloride secretion. Results indicate that the activation of either type I or type II PKA can maximally influence chloride secretion in T84 colonic epithelial cells.