A region in the Bacillus subtilis transcription factor SpoOA that is important for spoIIG promoter activation

A study was conducted to examine the role of single alanine substitution at the Bacillus subtilis transcription factor SpoOA on the activity of spoIIG promoters. The amino acid substitution in SpoOA which suppressed the phenotype of the H359R substitution in sigmaA subunit of the RNA polymerase showed that H359R substitution on sigmaA prevented its interaction with SpoOA. Results suggested the relevance of the region of SpoOA near position 229 in sigmaA-dependent promoter activation.