Physical-genetic map of the erythromycin-producing organism Saccharopolyspora erythraea

The restriction enzymes Asel and Dral were used to construct a physical and genetic map of the chromosome of the erythromycin-producing actinomycete Saccharopolyspora erythraea NRRL 2338. The digested fragments of the macrorestriction patterns of the restriction enzyme revealed a chromosome size of around 8Mb. It was also observed that 32% of the chromosome was covered by linking clones for the five contiguous Asel fragments. Moreover, the ribosomal RNA operons of S. erythraea contains an Asel site within the 16S(rrs) gene.