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Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma

Authors :
Collins, Colin
Rommens, Johanna M.
Kowbel, David
Godfrey, Tony
Tanner, Minna
Hwang, Soo-in
Polikoff, Daniel
Nonet, Genevieve
Cochran, Joanne
Myambo, Ken
Jay, Karen E.
Froula, Jeff
Cloutier, Thomas
Kuo, Wen-lin
Yaswen, Paul
Dairkee, Shanaz
Giovanola, Jennifer
Hutchinson, Gordon B.
Isola, Jorma
Kallioniemi, Olli-P
Palazzolo, Mike
Martin, Chris
Ericsson, Cheryl
Pinkel, Dan
Albertson, Donna
Li, Wu-bo
Gray, Joe W.
Source :
Proceedings of the National Academy of Sciences of the United States. July 21, 1998, Vol. 95 Issue 15, p8703, 6 p.
Publication Year :
1998

Abstract

We report here the molecular cloning of an [approximately equal to]1-Mb region of recurrent amplification at 20q13.2 in breast cancer and other tumors and the delineation of a 260-kb common region of amplification. Analysis of the 1-Mb region produced evidence for five genes, ZNF217, ZNF218, and NABC1, PIC1L (PIC1-like), CYP24, and a pseudogene CRP (Cyclophillin Related Pseudogene). ZNF217 and NABC1 emerged as strong candidate oncogenes and were characterized in detail. NABC1 is predicted to encode a 585-aa protein of unknown function and is overexpressed in most but not all breast cancer cell lines in which it was amplified. ZNF217 is centrally located in the 260-kb common region of amplification, transcribed in multiple normal tissues, and overexpressed in all cell lines and tumors in which it is amplified and in two in which it is not. ZNF217 is predicted to encode alternately spliced, Kruppel-like transcription factors of 1,062 and 1,108 aa, each having a DNA-binding domain (eight C2H2 zinc fingers) and a proline-rich transcription activation domain.

Details

ISSN :
00278424
Volume :
95
Issue :
15
Database :
Gale General OneFile
Journal :
Proceedings of the National Academy of Sciences of the United States
Publication Type :
Academic Journal
Accession number :
edsgcl.21033090