Origin of molecular species of diacylglycerol induced by bombesin in smooth muscle cells from rabbit rectosigmoid

A study was conducted to examine the origin of lipid sources of diacylglycerol production in rabbit smooth muscle cells based on contraction induced by peptide agonists. The animals' internal anal sphincter was removed by sharp dissection while smooth muscle cells were mixed with aliquots in glass tubes. Results indicated that bombesin-induced contraction by activation of phospholiase C led to the hydrolysis of various phospholipids.