Electrostatic stabilization in methionine aminopeptidase from hyperthermophile Pyrococcus furiosus

Authors :: Ogasahara, Kyoko
Lapshina, Elena A.
Sakai, Miyo
Izu, Yukiko
Tsunasawa, Susumu
Kato, Ikunoshin
Yutani, Katsuhide
Source :: Biochemistry. April 28, 1998, Vol. 37 Issue 17, p5939, 8 p.
Publication Year :: 1998
Abstract: A study was conducted to analyze the effects of salt on the thermal stability of a hyperthermophile Pyrococcus furiosus (PfMAP) using differential scanning calorimetry at various pHs. Enzymatic activities were determined in the presence of 1 mM CoCl2 at pH 7.0 while the gene of PfMAp was altered into Escherichia coli. Results indicated that electrostatic interaction is a critical factor in stabilizing the native form of PfMAP.

Subjects :: Calorimetry -- Usage
Salt -- Research
Electrostatics -- Research
Escherichia coli -- Research
Biological sciences
Chemistry

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