Back to Search
Start Over
Functional analysis of human RhCG: comparison with E. coli ammonium transporter reveals similarities in the pore and differences in the vestibule
- Source :
- The American Journal of Physiology. Sept, 2009, Vol. 297 Issue 3, pC537, 11 p.
- Publication Year :
- 2009
-
Abstract
- Rh glycoproteins are members of the ammonium transporter (Amt)/methylamine permease (Mep)/Rh family facilitating movement of NH3 across plasma membranes. Homology models constructed on the basis of the experimental structures of Escherichia coli AmtB and Nitrosomonas europaea Rh50 indicated a channel structure for human RhA (RhAG), RhB (RhBG), and RhC (RhCG) glycoproteins in which external and internal vestibules are linked by a pore containing two strictly conserved histidines. The pore entry is constricted by two highly conserved phenylalanines, 'twin-Phe.' In this study, RhCG function was investigated by stopped-flow spectrofluorometry measuring kinetic pH variations in HEK293E cells in the presence of an ammonium gradient. The apparent unitary NH3 permeability of RhCG was determined and was found to be close to that of AmtB. With a site-directed mutagenesis approach, critical residues involved in Rh N[H.sub.3] channel activity were highlighted. In the external vestibule, the importance of both the charge and the conformation of the conserved aspartic acid was shown. In contrast to AmtB, individual mutations of each phenylalanine of the twin-Phe impaired the function while the removal of both resulted in recovery of the transport activity. The impact of the mutations suggests that, although having a common function and a similar channel structure, bacterial AmtB and human Rh vary in several aspects of the N[H.sub.3] transport mechanisms. ammonium transport; HEK cells; Rhesus family, pH variations; ammonia channel
- Subjects :
- Escherichia coli -- Health aspects
Escherichia coli -- Genetic aspects
Escherichia coli -- Research
Glycoproteins -- Physiological aspects
Glycoproteins -- Genetic aspects
Glycoproteins -- Comparative analysis
Ion channels -- Physiological aspects
Ion channels -- Comparative analysis
Ion transport -- Physiological aspects
Ion transport -- Research
Biological sciences
Subjects
Details
- Language :
- English
- ISSN :
- 00029513
- Volume :
- 297
- Issue :
- 3
- Database :
- Gale General OneFile
- Journal :
- The American Journal of Physiology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.208640503