Genes for 2,4,5-trichlorophenoxyacetic acid metabolism in Burkholderia cepacia AC1100: characterization of the tftC and tftD genes and locations of the tft operons on multiple replicons

A study was conducted to determine and describe the tftCD gene to characterize the upper portion of the aromatic compound 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) pathway in Burkholderia cepacia. Plasmid-preparative columns were utilized to perform a large-scale purification of cosmids while agarose plugs with intact chromosomal DNA were determined from overnight cultures. Results showed that the genes encoding the 2,4,5-T oxygenase, tftAB, were on replicon IV.