Improved DNA extraction from ancient bones using silica-based spin columns

Authors :: Yang, Dongya Y.
Eng, Barry
Waye, John S.
Dudar, J. Christopher
Saunders, Shelley R.
Source :: American Journal of Physical Anthropology. April, 1998, Vol. 105 Issue 4, p539, 5 p.
Publication Year :: 1998
Abstract: We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick[TM], QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old. KEY WORDS DNA extraction; Ancient bone; PCR inhibitors; Silica-based spin columns