A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Authors :: Ptitsyn, L.R.
Horneck, G.
Komova, O.
Kozubek, S.
Krasavin, E.A.
Bonev, M.
Rettberg, P.
Source :: Applied and Environmental Microbiology. Nov, 1997, Vol. 63 Issue 11, p4377, 8 p.
Publication Year :: 1997
Abstract: A bioassay utilizing the SOS lux test was developed to detect environmental genotoxins rapidly. The assay is based on a luminescent bacterial gene combined to an SOS promoter. The recombinant gene is expressed in Escherichia coli. The presence of genotoxin in the environment induces a fluorescence in the recombinant bacteria. A dose-response relationship was observed during the testing of eight genotoxins.

Subjects :: Indicators (Biology) -- Research
Bacterial transformation -- Usage
Biological sciences

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