Transcriptional characterization of the Rickettsia prowazekii major macromolecular synthesis operon

A research study was conducted to analyze the Rickettsia prowazekii's (R prowazekii) gene organization and genetic transcription. The methods used were recombinant DNA technique and RNA technique. Results revealed that the genes found in R prowazekii are arranged into an operon. However, this gene organization seems different from that of the other bacteria. The open reading frame of the R prowazekii is also dissimilar from that of a gram-positive bacteria's open reading frames.