The eucaryal tRNA splicing endonuclease recognizes a tripartite set of RNA elements

Intron-containing tRNA precursors on both sides of the intron are divided by tRNA splicing endonuclease. Based on the experiment, the endonuclease uses different sets of structural elements which supersede the popular belief that the enzyme connects only to the mature domain by means of invariant bases. In the mature domain, a subset of recognition elements is necessary for recognition of both cleavage sites but in the exon-intron boundaries, two other subsets are utilized for recognition of either one or the other cleavage site. The findings advance the two-active model for eucaryal endonuclease.