Recombinant leptin exchanges between parabiosed mice but does not reach equilibrium

The circulating half-life of recombinant murine leptin was analyzed during parabiosis experiments involving ob/ob mutant mice that were deficient in circulating lipostatic signals. Quantitative analysis of the circulating half-life of recombinant murine leptin in ob/ob mice indicated the longer half-life of the protein in mice compared to humans. Furthermore, leptin was characterized as a parabiotic satiety signal protein that normalized serum insulin and glucose in ob/ob mice.