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Accelerated postnatal growth increases lipogenic gene expression adipocyte size in low-birth weight mice
- Source :
- Diabetes. May 2009, Vol. 58 Issue 5, p1192, 9 p.
- Publication Year :
- 2009
-
Abstract
- OBJECTIVE--To characterize the hormonal milieu and adipose gene expression in response to catch-up growth (CUG), a growth pattern associated with obesity and diabetes risk, in a mouse model of low birth weight (LBW). RESEARCH DESIGN AND METHODS--ICR mice were food restricted by 50% from gestational days 12.5-18.5, reducing offspring birth weight by 25%. During the suckling period, dams were either fed ad libitum, permitting CUG in offspring, or food restricted, preventing CUG. Offspring were killed at age 3 weeks, and gonadal fat was removed for RNA extraction, array analysis, RT-PCR, and evaluation of cell size and number. Serum insulin, thyroxine (T4), corticosterone, and adipokines were measured. RESULTS--At age 3 weeks, LBW mice with CUG (designated U-C) had body weight comparable with controls (designated C-C); weight was reduced by 49% in LBW mice without CUG (designated U-U). Adiposity was altered by postnatal nutrition, with gonadal fat increased by 50% in U-C and decreased by 58% in U-U mice (P < 0.05 vs. C-C mice). Adipose expression of the lipogenic genes Fasn, AccI, Lpin1, and Srebf1 was significantly increased in U-C compared with both C-C and U-U mice (P < 0.05). Mitochondrial DNA copy number was reduced by >50% in U-C versus U-U mice (P = 0.014). Although cell numbers did not differ, mean adipocyte diameter was increased in U-C and reduced in U-U mice (P < 0.01). CONCLUSIONS--CUG results in increased adipose tissue lipogenic gene expression and adipocyte diameter but not increased cellularity, suggesting that catch-up fat is primarily associated with lipogenesis rather than adipogenesis in this murine model.<br />Low-birth weight (LBW) infants are at increased risk for hypertension, type 2 diabetes, and metabolic syndrome (1). Mechanisms remain ill defined but may involve epigenetic regulation of development and gene [...]
- Subjects :
- Gene expression -- Research -- Genetic aspects -- Health aspects
Obesity in children -- Risk factors -- Genetic aspects -- Research -- Prevention
Birth weight, Low -- Health aspects -- Research
Type 2 diabetes -- Risk factors -- Prevention -- Genetic aspects -- Research
Health
Prevention
Research
Genetic aspects
Risk factors
Health aspects
Subjects
Details
- Language :
- English
- ISSN :
- 00121797
- Volume :
- 58
- Issue :
- 5
- Database :
- Gale General OneFile
- Journal :
- Diabetes
- Publication Type :
- Periodical
- Accession number :
- edsgcl.199463979