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In vitro assay for trans-phosphorylation of rhodopsin by rhodopsin kinase
- Source :
- Biochemistry. June 10, 1997, Vol. 36 Issue 23, p7064, 5 p.
- Publication Year :
- 1997
-
Abstract
- An assay for transphosphorylation of rhodopsin using split rhodopsin mutant SR(1-4/5-7), in which the protein was assembled in functional form from two separately expressed fragments, was devised. The use of SR(1-4/5-7) permits the biochemical distinction to be established for phosphorylated forms on the basis of electrophoretic mobility.
- Subjects :
- Rhodopsin -- Analysis
Enzymes -- Analysis
Biological sciences
Chemistry
Subjects
Details
- ISSN :
- 00062960
- Volume :
- 36
- Issue :
- 23
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.19788947