Hyperpolarization of hepatocytes by 2,5-AM: implications for hepatic control of food intake

The mechanisms that mediate the effects of 2,5-anhydro-D-mannitol (2,5-AM) on hepatocyte adenosine 3',5'-triphosphate (ATP) levels were analyzed in isolated mouse and rat liver cells based on a superfused liver slice method. Analysis of membrane potentials in superfused murine and rodent liver slices indicated the role of 2,5-AM in hyperpolarizing the hepatocyte cell membrane, reducing ATP levels and glucose production. Furthermore, 2,5-AM increased feeding by acting on metabolic sensors located in the hepatoportal area.