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ChIP-seq accurately predicts tissue-specific activity of enhancers
- Source :
- Nature. February 12, 2009, Vol. 457 Issue 7231, p854, 6 p.
- Publication Year :
- 2009
-
Abstract
- A major yet unresolved quest in decoding the human genome is the identification of the regulatory sequences that control the spatial and temporal expression of genes. Distant-acting transcriptional enhancers are particularly challenging to uncover because they are scattered among the vast non-coding portion of the genome. Evolutionary sequence constraint can facilitate the discovery of enhancers, but fails to predict when and where they are active in vivo. Here we present the results of chromatin immunoprecipitation with the enhancer-associated protein p300 followed by massively parallel sequencing, and map several thousand in vivo binding sites of p300 in mouse embryonic forebrain, midbrain and limb tissue. We tested 86 of these sequences in a transgenic mouse assay, which in nearly all cases demonstrated reproducible enhancer activity in the tissues that were predicted by p300 binding. Our results indicate that in vivo mapping of p300 binding is a highly accurate means for identifying enhancers and their associated activities, and suggest that such data sets will be useful to study the role of tissue-specific enhancers in human biology and disease on a genome-wide scale.<br />The initial sequencing of the human genome (1,2), complemented by effective computational and experimental strategies for mammalian gene discovery (3,4), has resulted in a virtually complete list of protein-coding sequences. [...]
- Subjects :
- Nucleotide sequencing -- Methods -- Usage -- Research
Gene expression -- Research -- Methods -- Usage
DNA sequencing -- Methods -- Usage -- Research
Human genome -- Research -- Methods -- Usage
Environmental issues
Science and technology
Zoology and wildlife conservation
Usage
Research
Methods
Subjects
Details
- Language :
- English
- ISSN :
- 00280836
- Volume :
- 457
- Issue :
- 7231
- Database :
- Gale General OneFile
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.194334487