Late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the entomopathogenic fungus Beauveria bassiana

Late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the insect pathogenic fungus Beauvria bassiana are examined by a combination of gene knockout, antisense RNA and gene coexpression studies. ORF2 has encoded an unusual cytochrome P450 monooxygenase needed for the selective N-hydroxylation of the 2-pyridone that is incapable of N-hydroxylation of acyltetramic acids.