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Assembly of the Ptdlns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3
- Source :
- The Journal of Cell Biology. Dec 15, 2008, Vol. 183 Issue 6, p1061, 14 p.
- Publication Year :
- 2008
-
Abstract
- The phosphoinositide phosphatidylinositol 4-phosphate (Ptdlns4P) is an essential signaling lipid that regulates secretion and polarization of the actin cytoskeleton. In Saccharomyces cerevisiae, the Ptdlns 4-kinase Stt4 catalyzes the synthesis of Ptdlns4P at the plasma membrane (PM). In this paper, we identify and characterize two novel regulatory components of the Stt4 kinase complex, Ypp1 and Efr3. The essential gene YPP1 encodes a conserved protein that colocalizes with Stt4 at cortical punctate structures and regulates the stability of this lipid kinase. Accordingly, Ypp1 interacts with distinct regions on Stt4 that are necessary for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches. We identify the membrane protein Efr3 as an additional component of Stt4 PIK patches. Efr3 is essential for assembly of both Yppl and Stt4 at PIK patches. We conclude that Yppl and Efr3 are required for the formation and architecture of Stt4 PIK patches and ultimately PM-based Ptdlns4P signaling.
Details
- Language :
- English
- ISSN :
- 00219525
- Volume :
- 183
- Issue :
- 6
- Database :
- Gale General OneFile
- Journal :
- The Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.191856600