Use of 13C-13C NOE for the assignment of NMR lines of larger labeled proteins at larger magnetic fields

The homonuclear dipolar interaction between adjacent 13C nuclei, otherwise known as the NOE method, is proposed as an alternative to the HCCH-COSY and HCCH-TOCSY techniques of studying large proteins in large magnetic fields. Unlike scalar transfer, magnetization transfer via NOE experiments increases the sensitivity and efficiency of experiments to assign the NMR lines despite the presence of relaxation mechanisms. The difference is approximately less than 20% for proteins with molecular weights of 30 kDa at 17.6 T.