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Use of 13C-13C NOE for the assignment of NMR lines of larger labeled proteins at larger magnetic fields
- Source :
- Journal of the American Chemical Society. Dec 11, 1996, Vol. 118 Issue 49, p12457, 2 p.
- Publication Year :
- 1996
-
Abstract
- The homonuclear dipolar interaction between adjacent 13C nuclei, otherwise known as the NOE method, is proposed as an alternative to the HCCH-COSY and HCCH-TOCSY techniques of studying large proteins in large magnetic fields. Unlike scalar transfer, magnetization transfer via NOE experiments increases the sensitivity and efficiency of experiments to assign the NMR lines despite the presence of relaxation mechanisms. The difference is approximately less than 20% for proteins with molecular weights of 30 kDa at 17.6 T.
- Subjects :
- Proteins -- Structure
Chemistry
Subjects
Details
- ISSN :
- 00027863
- Volume :
- 118
- Issue :
- 49
- Database :
- Gale General OneFile
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.19132430