Purification and characterization of two dihydroxyacetone kinases from Schizosaccharomyces pombe IFO 0354

The characterization of two dihydroxyacetone kinases (DHAKs), DHAK I and DHAK II, purified from Schizosaccharomyces pombe IFO 0354, shows that they are immunologically different from each other. DHAK II is more important than DHAK I for the dissimilation of glycerol via dihydroxyacetone. When the enzyme activity is measured in a mixed buffer, DHAK I shows optimum activity at pH 6.0 and DHAK II at pH 7.0. The optimum temperature for the activity of DHAK I is 50 degree celsius (C)and that for the activity for DHAK II is 60 degree C.