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Cloning, sequencing, and expression of isopropylbenzene degradation genes from Psuedomonas sp. strain JR1: identification of isopropylbenzene dioxygenase that mediates trichloroethene oxidation
- Source :
- Applied and Environmental Microbiology. Nov, 1996, Vol. 62 Issue 11, p3967, 11 p.
- Publication Year :
- 1996
-
Abstract
- The genes encoding enzymes involved in the catabolism of isopropylbenzene (IPB) in Psuedomonas sp. strain JR1 have been identified in a cosmid clone consisting of seven open reading frames. The ipbA gene codes for IPB dioxygenase, ipbB codes for 2,3-dihydro-2,3-dihydroxy-IPB dehydrogenase and ipbC codes for 3-isopropylcatechol 2,3-dioxygenase. The amino acid sequence of the clone is similar to that of the proteins involved in biphenyl-degradation in gram-positive and gram-negative bacteria. IPB is the inducer substrate for trichloroethene oxidation in Pseudomonas.
Details
- ISSN :
- 00992240
- Volume :
- 62
- Issue :
- 11
- Database :
- Gale General OneFile
- Journal :
- Applied and Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.19000585