Exploiting soft and hard X-ray absorption spectroscopy to characterize metallodrug/protein interactions: the binding of [trans-Ru[Cl.sub.4](lm)(dimethylsulfoxide)][ImH] (Im = imidazole) to bovine serum albumin

The studies on the reaction of bovine serum albumin (BSA) with [trans-Ru[Cl.sub.4](Im)(dimethylsulfoxide)][ImH] (Im = imidazole) (NAMI-A), an experimental ruthenium(III) anticancer drug and the formation of the respective NAMI-A/BSA adduct are presented. The results confirmed X-ray absorption spectroscopy to be a very suitable method for the characterization of metallodrug/protein adduct and nature and mechanism of its formation.