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Spectroscopic and protein chemical analyses demonstrate the presence of C-mannosylated tryptophan in intact human RNase 2 and its isoforms
- Source :
- Biochemistry. Sept 17, 1996, Vol. 35 Issue 37, p12005, 10 p.
- Publication Year :
- 1996
-
Abstract
- Mass spectrometric and nuclear magnetic resonance spectroscopic studies were performed on human urinary RNase 2 and its peptides at a pH of less than five. In addition, the effect of acidic conditions on the biosynthesis of the glycopeptide F(O3-Man-)TWA was examined. The results indicated that the C-mannosylation of Trp-7 in RNase 2 was not caused by the acidic conditions used in the isolation and purification of the enzyme and its peptides. In addition, RNase from erythrocites were also found to contain (C2-Man-)Trp.
Details
- ISSN :
- 00062960
- Volume :
- 35
- Issue :
- 37
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.18801509