Comparative total syntheses of turkey ovomucoid third domain by both stepwise solid phase peptide synthesis and native chemical ligation

Both the stepwise solid phase peptide synthesis technique and the native chemical ligation method were used in the total chemical synthesis of turkey ovomucolid third domain, (6-56)OMTKY3. Characterization of the resultant products showed that the two synthetic proteins are identical in function and structure to their recombinant counterpart. The two techniques also provided comparable yields and similar product quality.