Specificity analysis of three clonal and five non-clonal alpha-1,3-L-fucosyltransferases with sulfated, sialylated, or fucosylated synthetic carbohydrates as acceptors in relation to the assembly of 3'-sialyl-6'-sulfo Lewis x (the L-selectin ligand) and related complex structures

The cloned alpha-1,3-L-fucosyltransferases, FT III, FT IV, and FT V, have unique specificities for blood group type 2-based carbohydrate ligands and antigenic determinants. FT V participates more in the production of Lewis y than Lewis x. FT IV is very effective in synthesizing both 3'-sulfo Lewis x and Lewis x in mucin-type branched chains. Both FT III and FT V are more effective in synthesizing 3'-sulfo Lewis x than 3'-sialyl Lewis x. FT IV is more effective in synthesizing 6-sulfo Lewis x than the other two.