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Chromatin-bound mitogen-activated protein kinases transmit dynamic signals in transcription complexes in [beta]-cells

Authors :
Lawrence, Michael C.
McGlynn, Kathleen
Shao, Chunli
Duan, Lingling
Naziruddin, Bashoo
Levy, Marlon F.
Cobb, Melanie H.
Source :
Proceedings of the National Academy of Sciences of the United States. Sept 9, 2008, Vol. 105 Issue 36, p13315, 6 p.
Publication Year :
2008

Abstract

MAPK pathways regulate transcription through phosphorylation of transcription factors and other DNA-binding proteins. In pancreatic [beta]-cells, ERK1/2 are required for transcription of the insulin gene and several other genes in response to glucose. We show that binding of glucose-sensitive transcription activators and repressors to the insulin gene promoter depends on ERK1/2 activity. We also find that glucose and NGF stimulate the binding of ERK1/2 to the insulin gene and other promoters. An ERK1/2 cascade module, including MEK1/2 and Rsk, are found in complexes bound to these promoters. These findings imply that MAPK-containing signaling complexes are positioned on sensitive promoters with their protein substrates to modulate transcription in situ in response to incoming signals. c-fos | C/EBP-[beta] | ERK1/2 | hyperglycemia | insulin gene transcription

Details

Language :
English
ISSN :
00278424
Volume :
105
Issue :
36
Database :
Gale General OneFile
Journal :
Proceedings of the National Academy of Sciences of the United States
Publication Type :
Academic Journal
Accession number :
edsgcl.185386356