Determinants of cofactor specificity in isocitrate dehydrogenase: structure of an engineered NADP+ - NAD+ specificity-reversal mutant

Isocitrate dehydrogenase (IDH) is a decarboxylating dehydrogenase that is known to be involved with the Krebs Cycle, glutamic acid production and the provision of NADPH for biosyntheses. IDH, like many decarboxylating dehydrogenases, has been the subject of much research because its strong preference for either NAD or NADP has made it a target of enzyme redesign efforts. Determinants of IDH's cofactor specificity are examined using the structure of an engineered NADP - NAD specificity-reversal mutant as a reference point.