Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway

Maleylacetate reductase from Pseudomonas sp. strain B13 is one of the enzymes of the modified ortho pathway that is involved in chlorocatechol degradation. Purification and characterization of the enzyme by high-performance liquid chromatography detected tfdF and tcbF genes on plasmids pJP4 of Alcaligenes eutrophus and pP51 of Pseudomonas strain P51. These genes were shown to have significant similarities with those on plasmid pAC27 of Pseudomonas putida AC866.