CD9 antigen mRNA is induced by hypertonicity in two renal epithelial cell lines

Extracellular hypertonicity induces the formation of CD9 antigen mRNA in the renal epithelial cell lines Madin-Darby canine kidney and PAP-HT25. C9 is involved in the hyperosmotic stress response as hypertonicity increases the transcription of proteins, participating in metabolism and osmolyte transportation. The CD9 mRNA induction decreases with accumulation of organic osmolytes. Induction of C9 is specific rather than as a part of a generalized response system. Differential mRNA display polymerase chain reaction technique is used to detect the cDNA product of CD9 mRNA.