Physical map of the linear chromosome of Streptomyces griseus

A linear chromosome physical map of Streptomyces griseus 2247 was presented and its terminal construction was examined. The chromosome DNA was digested with various restriction endonucleases and studied by pulse-field gel electrophoresis (PFGE). Fifteen fragments from AseI and nine fragments from DraI were obtained. A 24-kb terminal inverted repeat was found on each end. PFGE analysis indicated the presence of a protein molecule at each end of the chromosome.