Anion secretion drives fluid secretion by monolayers of cultured human polycystic cells

Analysis of transepithelial characteristic of monolayers generated by primary cultures of human cystic epithelium reveals that an increase in active chloride anion secretion, controlled by adenosine 3',5'-cyclic monophosphate, causes fluid secretion by cystic epithelial cells. The secretion involves hyperpolarization of the apical-negative V(sub te) and an increase in positive I(sub sc). The experimental procedure of the analysis is discussed