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Lack of association between the -260 C[right arrow]T promoter polymorphism of the endotoxin receptor CD14 gene and the CD14 density of unstimulated human monocytes and soluble CD14 plasma levels

Lack of association between the -260 C[right arrow]T promoter polymorphism of the endotoxin receptor CD14 gene and the CD14 density of unstimulated human monocytes and soluble CD14 plasma levels

Authors :
Heesen, Michael
Blomeke, Brunhilde
Schluter, Bernhard
Heussen, Nicole
Rossaint, Rolf
Kunz, Dagmar
Source :
Intensive Care Medicine. Nov, 2001, Vol. 27 Issue 11, p1770, 6 p.
Publication Year :
2001

Abstract

Byline: Michael Heesen (1), Brunhilde Blomeke (2), Bernhard Schluter (3), Nicole Heussen (4), Rolf Rossaint (1), Dagmar Kunz (5) Keywords: CD14 antigen Genetics: gene polymorphism Molecular biology: real-time PCR Fluorescence-activated cell sorting Cytokines: tumor necrosis factor-[alpha] Abstract: Objective: CD14 is a receptor for endotoxin and binds components of Gram-positive and Gram-negative bacteria. CD14-bearing monocytes respond to stimulation with the increased synthesis and release of cytokines. The recently described --260 C[right arrow]T promoter polymorphism of the CD14 gene has been found to be related to a risk of myocardial infarction. This study evaluated the role of this polymorphism in the expression of monocyte and soluble CD14. Moreover, the effect of the CD14 --260 genotypes for the ex vivo TNF-[alpha] response to endotoxin was analyzed in whole blood. Patients and participants: Ninety-five healthy blood donors were studied. Measurements and results: CD14 --260 genotyping was performed by means of a real-time PCR with fluorescence labeled hybridization probes. CD14 expression on human monocytes (mCD14) was assessed by fluorescence-activated cell sorting analysis with anti-CD14 monoclonal antibodies. Plasma levels of soluble CD14 (sCD14) were measured by ELISA. The TNF-[alpha] synthesis was determined by chemiluminescence in whole blood after endotoxin stimulation. There were no differences in mCD14 density, sCD14 levels, or the tumor necrosis factor-[alpha] concentrations between individuals with the three different CD14 --260 genotypes CC, CT, and TT. Conclusions: The CD14 --260 polymorphism does not affect the CD14 expression of unstimulated circulating monocytes or soluble CD14 plasma levels. Author Affiliation: (1) Department of Anesthesiology and Intensive Care Medicine, University Hospital of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany (2) Department of Dermatology, University Hospital of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany (3) Department of Clinical Chemistry, University Hospital of Munster, Albert-Schweitzer-Strasse 33, 48149 Munster, Germany (4) Department of Statistics and Biometry, University Hospital of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany (5) Department of Clinical Chemistry and Pathobiochemistry, University Hospital of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany Article History: Received Date: 08/12/2000 Accepted Date: 21/08/2001 Article note: Final revision received: 21 August 2001 Electronic Publication

Details

Language :
English
ISSN :
03424642
Volume :
27
Issue :
11
Database :
Gale General OneFile
Journal :
Intensive Care Medicine
Publication Type :
Academic Journal
Accession number :
edsgcl.179867742