Human replication protein A melts a DNA triple helix structure in a potent and specific manner

The ability of purified endogenous human replication protein A (RPA) to melt DNA triplexes and tetraplexes was examined to show that RPA destabilizes a DNA tripe helix structure in a specific and highly effective manner. The results revealed that the large quantity of RPA known to exist in vivo might likely be a strong deterrent to the stability of triplexes that could potentially form from human genomic DNA sequences.