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DNA nanoswitch as a biosensor
- Source :
- Analytical Chemistry. June 15, 2007, Vol. 79 Issue 12, p4724, 5 p.
- Publication Year :
- 2007
-
Abstract
- We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 79
- Issue :
- 12
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.178894796