Structural characterization of the cytosolic domain of kidney chloride/bicarbonate anion exchanger 1 (kAE1)

The structural analysis of cytosolic domain (cdAE1), cytosolic domain kidney anion exchanger 1 (cdkAE1), and novel truncation mutant cd[Delta]54AE1 which lacks the first 54 residues but retains the core [beta]-strand is reported. The absence of the central [beta]-strand in cdkAE1 is found to result in a less stable and more open structure than cdAE1 and might account for the altered protein binding properties of kAE1 in addition to the loss of the acidic amino-terminal region.