Structural aligments of (+)- and (-)-trans-anti-benzo(a)pyrene-dG adducts positioned at a DNA template-primer function

The structural characteristics of (+) and (-)-trans-anti-benzo(a)-pyrene-dG adducts located at the junction site of single-strand/duplex 13/9-mer sequence were determined. The analyses involve the use of MNR spectrometry in combination with molecular mechanics calculations. The results showed that the modified 13-mer strand mimics the template strand, while the complementary 9-mer strand mimics a primer which extends from the 3'-end of the template toward the 5'-end up to the base preceding the modified guanine.