Enumeration of transconjugated Ruminococcus albus and its survival in the goat rumen microcosm

The mutant strain Ruminococcus albus A3 was introduced into the rumen of a goat and its survival was tested to determine how long antibiotic-resistant cellulose using bacteria can survive in the rumen. The number of bacteria decreased sharply after one day possibly because of the presence of protozoan predators and a low growth rate. In the rumen of the goat, the bacteria survived for 14 days, while in a simulated rumen, the bacteria survived for only 96 hours and the number of surviving cells was less.