HPCZE of nonionic compounds using a novel anionic surfactant additive

Excellent separations of nonionic organic compounds were obtained by adding sodium dioctyl sulfosuccinate (DOSS) to an acetonitrile ([approximately] 40% v/v)-water electrolyte. Separation is based on differences in the strength of analyte-DOSS association 'complexes' in solution, which results in differences in effective electrophoretic mobility. Micelle formation is not believed to occur. The effects of varying apparent pH, applied voltage, acetonitrile concentration, and DOSS concentration were studied with regard to electroosmotic mobility and effective electrophoretic mobility. Under optimum conditions, excellent separations of 23 organic compounds were obtained.