Indoleglycerol phosphate synthase - phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains

At a functional level, the bifunctional enzyme from Escherichia coli does not have any advantage over the synthesized monofunctional enzyme. The monomeric bifunctional enzyme indoleglycerol phosphate synthase: phosphoribosyl anthranilate isomerase was examined for field interaction through breaking up the domains on the gene level and creating monofunctional proteins. The synthesized monofunctional enzymes were stable and had all catalytic properties. There was no interaction between the enzymes, neither in vitro nor in vivo.