Molecular cloning and transcriptional analysis of the Aspergillus terreus gla1 gene encoding a glucoamylase

Heterologous hybridization facilitates the cloning of the Aspergillus terreus gla1 gene, which encodes a glucoamylase. Transcriptional analysis of the gene reveals the presence of a protein having a C-terminus starch-binding domain and N-terminal catalytic domain. Examination of the influence of carbon sources on gla1 regulation reveals that starch and maltose stimulate the expression of the gene, while glucose represses the expression.