Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius

Bioconversion, complementation and transcriptional analysis of the dnrI-aphII double mutant help study the function of dnrI, one of the two genes that constitute the two-component regulatory system, which regulates daunorubicin biosynthesis in Streptomyces peucetius. Delta-dnrJ mutant and wild-type strains possess all the transcripts that code for the early- and late-acting enzymes in the biosynthesis of daunorubicin, but are absent in the double mutant strain, indicating that dnrI regulates the biosynthetic genes. Transcriptional mapping of the 5' and 3' ends of dnrIJ transcript and the dnrZUV transcripts reveals that the two transcripts are complementary in the daunorubicin biosynthesis enzyme-encoding regions.